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Current Methods in Molecular and Microbiology

Module name (EN):
Name of module in study programme. It should be precise and clear.
Current Methods in Molecular and Microbiology
Degree programme:
Study Programme with validity of corresponding study regulations containing this module.
Engineering and Management, Master, SO 01.10.2024
Module code: MAM.2.1.6.20
Hours per semester week / Teaching method:
The count of hours per week is a combination of lecture (V for German Vorlesung), exercise (U for Übung), practice (P) oder project (PA). For example a course of the form 2V+2U has 2 hours of lecture and 2 hours of exercise per week.
2V+1P (3 hours per week)
ECTS credits:
European Credit Transfer System. Points for successful completion of a course. Each ECTS point represents a workload of 30 hours.
3
Semester: according to optional course list
Mandatory course: no
Language of instruction:
German
Assessment:
Exam

[updated 08.01.2024]
Applicability / Curricular relevance:
All study programs (with year of the version of study regulations) containing the course.

BMT2512.MBIO (P213-0156) Biomedical Engineering, Bachelor, ASPO 01.10.2018 , optional course, medical/technical, course inactive since 06.09.2022
BMT2512.MBIO (P213-0156) Biomedical Engineering, Bachelor, SO 01.10.2025 , optional course, medical/technical, course inactive since 06.09.2022
BMT1932 (P213-0156, P213-0164) Biomedical Engineering, Master, ASPO 01.04.2014 , optional course, general subject
MAM.2.1.6.20 Engineering and Management, Master, ASPO 01.10.2019 , optional course, general subject
MAM.2.1.6.20 Engineering and Management, Master, SO 01.10.2024 , optional course, general subject
MP2208.MBIO (P213-0156) Medical Physics, Master, ASPO 01.04.2019 , semester 2, optional course, medical/technical
MP2208.MBIO (P213-0156) Medical Physics, Master, SO 01.10.2025 , semester 2, optional course, medical/technical
Workload:
Workload of student for successfully completing the course. Each ECTS credit represents 30 working hours. These are the combined effort of face-to-face time, post-processing the subject of the lecture, exercises and preparation for the exam.

The total workload is distributed on the semester (01.04.-30.09. during the summer term, 01.10.-31.03. during the winter term).
45 class hours (= 33.75 clock hours) over a 15-week period.
The total student study time is 90 hours (equivalent to 3 ECTS credits).
There are therefore 56.25 hours available for class preparation and follow-up work and exam preparation.
Recommended prerequisites (modules):
None.
Recommended as prerequisite for:
Module coordinator:
Prof. Dr. Timo Gehring
Lecturer: Prof. Dr. Timo Gehring

[updated 15.10.2021]
Learning outcomes:
After successfully completing this course, students will have significantly increased their knowledge in molecular biology, microbiology and hygiene. They will be proficient in the molecular biological methods currently used in clinical practice and research that enable a better understanding of modern medical diagnostic procedures and biotechnological processes.
Students will be familiar with the most important pathogenic bacteria and viruses, as well as antibiotics and the growing problem of antibiotic resistance. They will be able to assess which disinfection and sterilisation procedures should be used and when. They will be familiar with biotechnological processes and be able to cultivate the algae that also play a role in the pharmacological field.


[updated 08.01.2024]
Module content:
Basics of molecular biology
Current molecular biological and microbiological methods used for clinical diagnosis and in biotechnology.
Molecular biological methods:
•        Polymerase Chain Reaction (PCR)
•        DNA-modifying methods (e.g. restriction digestion, CRISPR-CAS, detailed implementation of cloning)
•        Southern blot analyses, DNA microarray and chip technology
•        DNA sequencing (Sanger method and Next Generation Sequencing)
 
Micro-biological procedures
•        Medical microbiology (pathogenic bacteria, viruses and others)
•        Hospital infections
•        Antibiotics and development of resistance against antibiotics
•        Desinfection and sterilization
•        Photosynthesis and the use of algae in biotechnology
 
Safety seminar for the practical course
Use of the following molecular biological/biotechnological methods in the practical course:
•        Isolating DNA from various algae (cyanobacteria)
•        Amplification of 16S rDNA of these cyanobacteria by PCR
•        DNA gel electrophoresis


[updated 08.01.2024]
Teaching methods/Media:
Video projector, Powerpoint presentation, lab experiments

[updated 08.01.2024]
Recommended or required reading:
Biochemie - H.R. Horton, L.A. Moran, K.G. Scrimgeour, M.D. Perry, J.D. Rawn.
Brock Mikrobiologie - M.T. Madigan, J.M. Martinko.
Hygiene und medizinische Mikrobiologie - M. Dülligen, A. Kirov, H. Unverricht.

[updated 08.01.2024]
[Mon Dec 23 02:31:50 CET 2024, CKEY=bamdmum, BKEY=mm3, CID=MAM.2.1.6.20, LANGUAGE=en, DATE=23.12.2024]